APC Anti-Mouse CD49f Monoclonal Antibody for FC - Q61739

Core Product Specifications and Parameters

Biological Background: Integrin alpha-6 Function and Localization

• Integrin alpha-6 (ITGA6, CD49f) is a transmembrane glycoprotein that heterodimerizes with integrin beta-1 (ITGB1) or beta-4 (ITGB4) to form receptors for laminin.
• ITGA6:ITGB1 mediates platelet adhesion to laminin. Related references: PMID:8081870
• ITGA6:ITGB1 is critical for sperm-egg fusion during fertilization. Related references: PMID:10634791
• ITGA6:ITGB4 anchors epithelial cells to the basement membrane via hemidesmosomes. Related references: PMID:8673141
• The ITGA6:ITGB4 complex also binds growth factors (NRG1, IGF1, IGF2) to modulate ERBB and IGF1R signaling pathways.
• ITGA6 localizes to the cell membrane and exists in multiple isoforms due to alternative splicing.
• Post-translational modifications such as palmitoylation, phosphorylation, and cleavage on basic residues regulate its function.
• In mouse, Itga6 expression is low in normal skin but increases in skin tumors, suggesting a role in tumor progression.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is ready-to-use for direct flow cytometry applications. Consider titrating the antibody to determine the optimal concentration for your specific sample type.
• Since the immunogen is a synthetic peptide derived from mouse CD49f, the antibody is expected to recognize mouse and human integrin alpha-6. It is advisable to validate cross-reactivity in the relevant species and tissue.
• For surface staining, ensure cells are viable and use appropriate isotype controls to distinguish specific signal. For intracellular epitopes, a fixation and permeabilization step may be required, though the epitope location should be considered.
• Target cells known to express ITGA6 include platelets, oocytes, epithelial cells, and certain tumor cells. Confirm expression in your system using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies like this APC anti-CD49f conjugate, and ancillary reagents to support cell adhesion and integrin research. Whether you need bulk quantities or rare-target raw materials for assay development, we are your sourcing bridge from concept to clinic.