APC Anti-Mouse CD44 Monoclonal Antibody for FC - P15379

Core Product Specifications and Parameters

Biological Background: Mouse CD44 Function and Localization

• CD44 is a cell-surface receptor for hyaluronan (HA) and other extracellular matrix components, playing a central role in cell-cell and cell-matrix adhesion, leukocyte homing, and recirculation.
  Related references:
  PMID:25065622 PMID:8343954
• Through its cytoplasmic domain, CD44 recruits signaling complexes containing RhoGTPases (RAC1, RHOA) and kinases (PKN2), regulating actin cytoskeleton reorganization and cell migration.
  Related references:
  PMID:25065622 PMID:8343954
• CD44 exists in multiple isoforms generated by alternative splicing, and is heavily modified post-translationally by glycosylation, sulfation, and phosphorylation, influencing ligand binding and signaling.
• The protein is predominantly localized to the plasma membrane and microvilli, but a secreted form is also produced; in mice, it is notably expressed in the intestinal epithelium.
• Engagement of CD44 with galectin-9 (LGALS9) activates LCK/ERK/MAPK signaling, enhancing natural killer (NK) cell cytotoxic activity.
  Related references:
  PMID:37006235
• CD44 participates in inflammatory responses and defense against bacterial infection by facilitating immune cell adhesion and extravasation into infected tissues.
  Related references:
  PMID:25065622 PMID:8343954

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is designed for flow cytometry; titrate the antibody to determine the optimal concentration for your specific experimental setup and use appropriate isotype controls to confirm specificity.
• Since the immunogen covers the extracellular region (aa 23-176), the antibody is expected to recognize cell-surface CD44; fixable viability dyes may be used in combination for live/dead discrimination.
• CD44 is expressed on many leukocyte subsets; validate staining in your target population and consider blocking Fc receptors to reduce background.
• Isoform-specific expression varies across tissues; if you need to distinguish CD44 variants, complement with isoform-specific detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and laboratories with comprehensive access to high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies targeting CD44 and other immunology/inflammation markers. Our one-stop service model supports your entire development workflow, from bulk ancillary reagent supply to rare-target sourcing, helping you accelerate assay development with confidence.