APC Anti-Mouse CD19 Monoclonal Antibody for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• B-lymphocyte antigen CD19, also known as Differentiation antigen CD19, is a cell surface protein specifically expressed on B cells.
• Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes, lowering the threshold for activation of downstream signaling pathways and triggering B-cell responses to antigens.
• Activates signaling pathways that lead to phosphatidylinositol 3-kinase activation and the mobilization of intracellular Ca(2+) stores. Related references: PMID:12387743, PMID:20101619, PMID:9382888
• Not required for early steps during B cell differentiation in bone marrow, but essential for normal differentiation of B-1 cells. Related references: PMID:7542548, PMID:7543183, PMID:9317126
• Required for normal B cell differentiation and proliferation in response to antigen challenges. Related references: PMID:12387743, PMID:7542548, PMID:9317126
• Required for normal levels of serum immunoglobulins and for production of high-affinity antibodies in response to antigen challenge. Related references: PMID:12387743, PMID:7542548, PMID:7543183
• Subcellular location: Localizes to the cell membrane and is enriched in membrane rafts.
• Tissue specificity: Detected on B cells in spleen, bone marrow, thymus, and lymph nodes, and on peripheral blood lymphocytes (at protein level). Related references: PMID:12387743, PMID:20101619, PMID:7542548, PMID:7543183
• Belongs to the immunoglobulin superfamily, possessing multiple immunoglobulin-like domains, disulfide bonds, and phosphorylation sites (glycoprotein, phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19–287), making the antibody suitable for detecting surface-expressed CD19 on live B cells.
• Directly conjugated to APC, it is ready for flow cytometry without a secondary antibody; consider titrating to optimize signal-to-noise in your specific cell population.
• Validate performance in relevant mouse B-cell samples (splenocytes, bone marrow, lymph node cells, peripheral blood) with appropriate isotype and compensation controls.
• For multicolor panels, ensure minimal spectral overlap with other fluorophores and include fluorescence-minus-one (FMO) controls for accurate gating.
• Since CD19 expression persists throughout B-cell development, it can serve as a pan-B-cell marker across differentiation stages.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high-quality APC-conjugated CD19 monoclonal antibody as part of a full portfolio of IVD raw materials for B-cell research and diagnostic development. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay manufacturing. Whether you need rare target sourcing or custom conjugation services, CamelBio bridges the gap from concept to clinic with reliable, scalable supply solutions.