APC Anti-Mouse CD11c Monoclonal Antibody for Flow Cytometry - Q9QXH4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X Function and Localization

• Integrin alpha-X (CD11c) is a type I transmembrane glycoprotein that pairs with integrin beta-2 to form the CD11c/CD18 heterodimer, also known as p150,95.
• This integrin serves as a receptor for fibrinogen, specifically recognizing the G-P-R sequence, and mediates cell-cell adhesion during inflammatory responses.
• It plays a crucial role in monocyte adhesion and chemotaxis, facilitating leukocyte migration to sites of inflammation.
• Additionally, CD11c functions as a receptor for ICAM4 on erythrocytes, mediating erythrophagocytosis.
• Subcellular localization is primarily at the cell membrane, consistent with its role in adhesion and signaling.
• The protein contains multiple domains including calcium-binding repeats, a transmembrane helix, and disulfide bonds, classifying it as a metal-binding integrin receptor.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant protein immunogen, which may target a specific domain or full-length protein. Consider epitope mapping if precise binding site information is required.
• For flow cytometry applications, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and ensure proper compensation when using APC conjugates in multicolor panels.
• Validate performance in your specific mouse sample system, and be aware that expression levels can vary among cell subsets and activation states.
• Given the protein weight of 129 kDa, ensure adequate fixation and permeabilization if intracellular staining is performed, though this product is validated only for surface FC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-CD11c antibody facilitates research into monocyte adhesion and inflammatory responses, and CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target raw materials to support your assay development—from concept to clinic.