APC Anti-Integrin alpha 5 (ITGA5/CD49e) Monoclonal Antibody for Flow Cytometry - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 (ITGA5/CD49e) Function and Localization

• Integrin alpha-5 (CD49e, fibronectin receptor subunit alpha, VLA-5) is a key adhesion receptor. It forms heterodimers with integrin beta-1 (ITGB1) to mediate cell attachment to fibronectin, fibrinogen, and fibrillin-1 via recognition of the RGD sequence. ITGA5:ITGB1 also serves as a receptor for interleukin-1 beta (IL1B) and, together with ITGB3, participates in CD40/CD40LG signaling. Additionally, the integrin interacts with PLA2G2A to modulate its own ligand‑binding activity.
  Related references:
  PMID:18635536 PMID:25398877 PMID:12807887 PMID:17158881 PMID:33962943 PMID:29030430 PMID:31331973

• The protein localizes to the cell membrane, cell‑cell junctions, and focal adhesions, consistent with its role in cell adhesion and migration.

• Tissue expression profiling shows detectable levels of ITGA5 in placenta at the protein level.

• Integrin alpha-5 undergoes several post‑translational modifications including cleavage on a pair of basic residues, phosphorylation, disulfide bond formation, and N‑linked glycosylation. It contains calcium‑binding motifs essential for integrin function.

• ITGA5 is implicated in microbial pathogenesis: it acts as a cellular receptor for human metapneumovirus, human parvovirus B19 (via the ITGA2:ITGB1 complex), and HIV‑1 Tat protein in Kaposi’s sarcoma lesions.

• The mature protein has a predicted molecular weight of approximately 114.5 kDa (UniProt), consistent with the observed band around 115 kDa in gel electrophoresis.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 42‑995) of human ITGA5, suggesting the antibody likely recognizes an extracellular epitope. This makes it well‑suited for flow cytometry applications where surface staining of live cells is desired.
• The antibody is directly conjugated to APC, eliminating the need for a secondary antibody step in FC assays. Verify instrument compatibility with the APC fluorophore and perform an optimal titration to minimize background.
• Reported cross‑reactivity with both human and mouse ITGA5 allows for potential use in xenograft or mouse model studies; however, independent validation in the relevant sample system is recommended.
• For flow cytometry, include appropriate isotype controls and use a viability dye to exclude dead cells. Consider testing in both native and recombinant expression systems to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high‑quality IVD raw materials. Beyond this APC‑conjugated anti‑integrin alpha‑5 monoclonal antibody, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare‑target raw materials to accelerate your assay development from concept to clinic. Contact us to discuss how we can support your cell adhesion or receptor research programs with reliable, scalable supply.