APC Anti-Human CD335/NKp46 Monoclonal Antibody for Flow Cytometry - O76036

Core Product Specifications and Parameters

Biological Background: CD335/NKp46 Function and Localization

• Natural cytotoxicity triggering receptor 1 (NCR1, also known as CD335 and NKp46) is a type I transmembrane glycoprotein that serves as a key activating receptor on natural killer (NK) cells. It is encoded by the NCR1 gene (Gene ID: 9437) in humans.
• CD335/NKp46 functions as a cytotoxicity-activating receptor, enhancing the ability of activated NK cells to mediate tumor cell lysis.
• The receptor is selectively expressed on the surface of both resting and activated NK cells, making it a specific marker for NK cell identification.
• Multiple protein isoforms are generated through alternative splicing, resulting in calculated molecular weights ranging from approximately 21 kDa to 34 kDa.
• Subcellular localization is primarily at the cell membrane, with the receptor featuring an extracellular region containing two immunoglobulin-like domains, a transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for proper protein folding and function.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to allophycocyanin (APC) and designed for flow cytometry. Optimize the working concentration by titration using human NK cell lines or primary NK cells.
• Since the immunogen corresponds to the extracellular domain (amino acids 22–254), the antibody should recognize the native receptor on live, unfixed cells. Consider cell surface staining protocols.
• Use an appropriate isotype control (e.g., rabbit IgG-APC) to assess non-specific binding, especially in samples with high Fc receptor expression.
• The antibody is validated for human reactivity; cross-reactivity with other species has not been tested. Validate independently if applying to non-human samples.
• For multicolor flow cytometry panels, check spectral overlap and compensate properly, as APC emits in the far-red channel.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers and research laboratories with a single point of access to high-quality IVD raw materials, from concept to clinic. This anti-CD335/NKp46 antibody supports research into NK cell biology and tumor immunology.
• Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare-target raw materials, helping streamline your assay development and procurement workflows.