APC Anti-Human CD24 Monoclonal Antibody for Flow Cytometry - P25063

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24, also known as signal transducer CD24 or small cell lung carcinoma cluster 4 antigen, is a GPI-anchored glycoprotein encoded by the CD24 gene (aliases: CD24A).
• May have a pivotal role in cell differentiation of different cell types, with signaling triggered by lectin-like ligand binding to CD24 carbohydrates and transduced by GPI-anchor-derived second messengers.
• Modulates B-cell activation responses: promotes antigen-dependent proliferation of B-cells and prevents their terminal differentiation into antibody-forming cells. Related references: PMID:11313396
• In association with SIGLEC10, may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90, and plays a role in the control of autoimmunity (By similarity).
• Localized to the cell membrane via a GPI-anchor.
• Expressed on B-cells, a number of B-cell lines (P32/ISH, Namalwa), T-cells, erythroleukemia cell lines, and small cell lung carcinoma cell lines.
• Computed molecular weight of the unprocessed precursor is approximately 8 kDa; glycosylated mature forms migrate around 13 kDa.
• Features alternative splicing, and is a glycoprotein, lipoprotein, and GPI-anchored membrane protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1–80) of human CD24, which contains the mucin-like domain; this region is likely accessible for flow cytometry detection on live cells.
• For flow cytometry applications, consider titrating the antibody to determine the optimal concentration for your specific cell type, and include appropriate isotype controls.
• CD24 expression is predominantly on B-cells and certain cancer cell lines; validate antibody performance in your relevant sample system.
• This product ships on ice bags; upon receipt, store according to the manufacturer’s recommendations to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD antibodies, including validated monoclonal antibodies like this anti-CD24 clone, suitable for flow cytometry applications in immune profiling and cancer research. Beyond individual reagents, we offer tailored sourcing of antibody pairs, bulk ancillary materials, and rare‑target raw materials to streamline your assay development from concept to clinic.