APC Anti-Human CD101/IGSF2 Monoclonal Antibody for Flow Cytometry - Q93033

Core Product Specifications and Parameters

Biological Background: CD101/IGSF2 Function and Localization

• CD101, also known as immunoglobulin superfamily member 2 (IGSF2) or cell surface glycoprotein V7 (EWI-101), is a single-pass type I membrane protein belonging to the immunoglobulin superfamily.
• It functions as a negative regulator of T-cell activation, inhibiting CD3-induced proliferation, IL2RA expression, and IL2 secretion through the suppression of tyrosine kinases and phospholipase C-gamma-1 phosphorylation.
• CD101 prevents the nuclear translocation of NFAT, thereby blocking downstream calcium signaling and cytokine production in T-cells.
• Additionally, CD101 contributes to the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells, indicating a role in peripheral tolerance.
• The protein is widely expressed in lung, thymus, and small intestine, and is detected on cutaneous dendritic cells, activated T-cells, monocytes, granulocytes, and certain leukemic cells (e.g., CD4+ CD56+ blastic tumor cells).
• Subcellularly, CD101 is localized to the membrane and contains multiple immunoglobulin-like domains, a transmembrane helix, disulfide bonds, and glycosylation sites.
• Given its immunomodulatory functions, CD101 may serve as a marker for specific dendritic cell subsets and leukemic tumor cells, with potential relevance in immuno-oncology and autoimmune disease research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 21-410) of human CD101, making this antibody suitable for detecting surface-expressed CD101 on live cells by flow cytometry.
• Since CD101 is expressed on activated T-cells but not on resting T-cells, consider using in vitro–activated human peripheral blood mononuclear cells (PBMCs) or cell lines such as Jurkat (with appropriate stimulation) as positive controls.
• The provided APC conjugate allows direct detection without a secondary antibody; typical starting titrations for flow cytometry range from 0.5 to 5 µg/mL, but optimal concentration must be determined empirically.
• For intracellular staining or analysis of CD101 cleavage products, additional validation may be required, as this antibody targets the extracellular region.
• Given the relative molecular mass of approximately 115 kDa, confirm full-length CD101 expression by western blot if needed, though this product is primarily validated for flow cytometry.

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