APC Anti-F4/80 Monoclonal Antibody for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: Adhesion G protein-coupled receptor E1 (F4/80) Function and Localization

• Adhesion G protein-coupled receptor E1, also known as F4/80, EMR1, or Ly71, is a member of the adhesion GPCR family characterized by a long extracellular domain with EGF-like repeats.
• It functions as an orphan receptor likely involved in cell adhesion and cell-cell interactions, particularly within the immune system, and may play a role in regulatory T-cell (Treg) development.
• The protein is highly expressed on the surface of macrophages, with low-level expression on blood monocytes; it is absent from macrophages localized within T-cell areas of lymph nodes and spleen.
• Subcellularly, F4/80 is localized at the cell membrane, consistent with its role as a transmembrane receptor and surface marker.
• Key structural features include multiple EGF-like domains, glycosylation sites, disulfide bonds, and a GPCR proteolysis site, classifying it as a G protein-coupled receptor transducer.
• The predicted molecular weight is approximately 102 kDa, though glycosylation may increase the apparent weight in experimental systems.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular region (amino acids 28-644) of mouse F4/80, which is appropriate for generating antibodies targeting the extracellular domain for live-cell flow cytometry.
• This APC-conjugated antibody is designed for direct flow cytometric detection of F4/80 in mouse samples; ensure appropriate controls and optimize staining conditions for your specific cell type.
• Given the protein's high macrophage specificity, consider using this antibody for identifying murine macrophages in mixed populations; confirm specificity by including relevant positive and negative controls.
• For optimal performance, store the antibody at 2-8°C protected from light and avoid freeze-thaw cycles to preserve conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material provider offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. The APC Anti-F4/80 monoclonal antibody supports immunological research and diagnostic applications focusing on macrophage identification and characterization. With our one-stop sourcing model, we can assist in scaling your projects from concept to clinic, including rare target sourcing and technical consultation.