APC Anti-CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• Interleukin-2 receptor subunit alpha (CD25), also known as p55, is encoded by the Il2ra gene in mice and is a key component of the high-affinity IL-2 receptor, essential for proper immune homeostasis.
• CD25 is constitutively expressed at high levels on regulatory T cells (TREGs) and is critical for their function in maintaining immune tolerance by suppressing activation and expansion of autoreactive T-cells.
• The protein is a single-pass type I membrane glycoprotein with an extracellular region containing two sushi (CCP/SCR) domains that facilitate ligand binding and receptor complex formation.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are important for correct folding and stability of the extracellular domain.
• Subcellular localization is primarily at the plasma membrane, with a short cytoplasmic tail involved in signal transduction upon IL-2 engagement.
• CD25 is also transiently upregulated on activated conventional T cells, B cells, and NK cells, serving as an early activation marker.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 22-236) of mouse CD25, which is expected to generate antibodies recognizing the native surface receptor, well-suited for flow cytometry applications.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions, using relevant positive (e.g., activated T cells) and negative controls.
• Surface staining can be performed on live, unfixed cells; avoid fixation with reagents that may alter the APC fluorophore or epitope accessibility unless validated.
• Consider co-staining with markers such as Foxp3 (intracellular) or CD4 to identify Treg subsets or activated T cells in murine samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to premium IVD raw materials. This APC-conjugated anti-mouse CD25 monoclonal antibody caters to applications in immune monitoring and autoimmune disease research, backed by our comprehensive supply chain for validated antibody pairs, bulk ancillary reagents, and rare target sourcing. From concept to clinic, CamelBio bridges your development needs with efficient, end-to-end procurement solutions in the IVD space.