APC Anti-CD158a/KIR2DL1 Monoclonal Antibody for FC - P43626

Core Product Specifications and Parameters

Biological Background: CD158a/KIR2DL1 Function and Localization

• CD158a/KIR2DL1 is a killer cell immunoglobulin-like receptor (KIR) also known as NKAT-1, p58.1, and CD158A, belonging to the immunoglobulin superfamily.
• It functions as an inhibitory receptor on natural killer (NK) cells, recognizing specific HLA-C alleles (such as w4 and w6) and suppressing NK cell-mediated cytotoxicity to prevent lysis of target cells.
• The receptor is a type I transmembrane glycoprotein composed of two extracellular immunoglobulin-like domains (D1 and D2), a transmembrane helix, and a cytoplasmic tail containing ITIM motifs for inhibitory signaling.
• KIR2DL1 is a member of the polymorphic KIR gene family on chromosome 19, which includes both activating and inhibitory receptors that fine-tune innate immune responses.
• Subcellular localization is primarily at the cell membrane, where it interacts with MHC class I molecules on target cells.
• Alternative splicing generates multiple isoforms, and the protein undergoes post-translational modifications including disulfide bond formation and N-linked glycosylation.
• Expression is largely restricted to NK cells, though trace expression may be found on subsets of T cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 22-245), encompassing the two immunoglobulin-like domains, suggesting the antibody likely recognizes an epitope in this region.
• Given the APC conjugate and specificity for human CD158a, consider titrating the antibody for optimal resolution in flow cytometry, especially for rare NK cell subsets.
• For flow cytometry, stain live cells in appropriate buffer; avoid fixation that may alter epitope conformation unless validated in the relevant sample system.
• Cross-reactivity with other KIR family members (e.g., KIR2DL3) should be assessed if simultaneous staining is planned, as sequence homology exists.
• Although rated for human reactivity, verify performance in the exact cell type (e.g., primary NK cells, NK cell lines) and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports immune profiling and NK cell studies with reliable anti-KIR reagents like this CD158a/KIR2DL1 monoclonal antibody. We streamline sourcing from lead identification to clinical assay development, helping you accelerate diagnostic workflows.