APC Anti-CD141/Thrombomodulin Monoclonal Antibody for Flow Cytometry - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin Function and Localization

• Endothelial cell receptor that plays a critical role in regulating hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Related references: PMID:10761923
• Acts as a cofactor for thrombin activation of protein C/PROC, initiating the activated protein C anticoagulant pathway on vascular endothelial cells. Related references: PMID:29323190, PMID:33836597, PMID:9395524
• Accelerates activation of plasma carboxypeptidase B2/CPB2, removing C-terminal basic amino acids from kinins or anaphylatoxins, thereby inhibiting fibrinolysis. Related references: PMID:26663133
• Modulates protease-activated receptor 1/PAR1 cleavage specificity, contributing to the inhibition of endothelial cell permeability and inflammation.
• Suppresses inflammation independently of its anticoagulant cofactor activity by sequestering HMGB1, preventing its engagement with pro-inflammatory receptors like RAGE. Related references: PMID:15841214
• Localized to the plasma membrane; synthesized almost exclusively by endothelial cells.
• Structural hallmarks include EGF-like domains, multiple disulfide bonds, and glycosylation; molecular weight approximately 60 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your target cell type; start with 0.1–1 µg per test as a reference range.
• The APC conjugate enables direct detection, eliminating the need for a secondary antibody and simplifying multicolor panel design.
• Since the immunogen encompasses the entire extracellular region (aa 19-515), the antibody is expected to recognize the native cell surface receptor; validate expression using human endothelial cells or CD141‑positive cell lines.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and data interpretation.

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CamelBio delivers a complete range of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, supporting diagnostic manufacturers and research laboratories worldwide. For coagulation and inflammation targets like thrombomodulin, we ensure consistent supply and technical guidance. Trust CamelBio as your one‑stop sourcing partner, bridging the gap from concept to clinic with reliable products and expert consulting.