APAF1 Rabbit pAb for WB, IF/ICC, ELISA - O14727

Core Product Specifications and Parameters

Biological Background: APAF1 Function and Localization

• APAF1 encodes the apoptotic protease-activating factor 1 (Apaf-1), a cytoplasmic protein that plays a central role in the intrinsic apoptosis pathway.
• Upon cytochrome c release from mitochondria, Apaf-1 oligomerizes and, in an ATP-dependent manner, recruits and activates pro-caspase-9 (Apaf-3), triggering downstream caspase-3 activation and apoptosis.
• Isoform 6 of APAF1 is known to be less effective in inducing apoptosis compared to the canonical isoform.
• APAF1 is ubiquitously expressed, with highest mRNA levels in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney, and lung. Isoform 1 is detected in heart, kidney, and liver.
• APAF1 is classified under keywords including apoptosis, ATP-binding, nucleotide-binding, calcium, WD repeat, and alternative splicing, reflecting its functional and structural complexity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 470–600 of human APAF1 (NP_863651.1), a region that may be conserved across isoforms; consider verifying isoform specificity in your system.
• For Western blot (WB), multiple bands may be observed due to APAF1 isoforms and potential post-translational modifications; the predicted molecular weight range is 38–142 kDa. Validate band identification using appropriate controls.
• This polyclonal antibody is suitable for WB, IF/ICC, and ELISA applications as indicated; for each assay, optimize antibody dilution and detection conditions in the relevant sample matrix.
• Given the cross-reactivity with human, mouse, and rat, this antibody can be used for comparative studies across these species; confirm cross-reactivity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing diagnostic manufacturers and researchers with seamless access to validated antibodies, bulk ancillary reagents, and customized sourcing solutions. For apoptosis research and assay development, we offer a comprehensive portfolio including this APAF1 antibody and complementary reagents to support your workflow from concept to clinic. Contact us to discuss your specific procurement needs and capitalize on our B2B supply chain expertise.