AP3B1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O00203

Core Product Specifications and Parameters

Biological Background: AP3B1 Function and Localization

• AP3B1 encodes the AP-3 complex subunit beta-1, also known as beta-3A-adaptin, which is a large subunit of the adaptor protein complex 3 (AP-3).
• AP-3 functions as a non-clathrin- and clathrin-associated adaptor complex in the late-Golgi/trans-Golgi network (TGN) and endosomes, mediating clathrin recruitment to membranes and recognition of sorting signals within cytosolic tails of transmembrane cargoes.
• The complex is critically involved in sorting a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles.
• In concert with the BLOC-1 complex, AP-3 is required for targeting cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
• Subcellularly, AP-3 localizes to cytoplasmic vesicles, clathrin-coated vesicle membranes, and the Golgi apparatus.
• AP3B1 is ubiquitously expressed across tissues.
• Mutations in AP3B1 are associated with Hermansky-Pudlak syndrome type 2 (HPS2), characterized by albinism and bleeding diathesis.
• The protein is subject to phosphorylation, and multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 121 kDa; use an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required to unmask the epitope; validate staining with known positive and negative tissue controls.
• As a polyclonal antibody, it may recognize multiple isoforms or other AP-3 adaptor beta subunits; confirm specificity in your experimental system using appropriate knockdown or knockout models.
• The immunogen corresponds to amino acids 895–1094 of human AP3B1, a region with potential sequence conservation across species; cross-reactivity with mouse and rat is expected based on reported reactivity.
• Include recommended controls (e.g., lysate from cells with high AP3B1 expression, peptide blocking) to verify signal specificity in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with comprehensive access to IVD raw materials, technical services, and consulting. For AP3B1, a key adaptor protein implicated in lysosomal trafficking and Hermansky-Pudlak syndrome, we offer validated antibody reagents and can assist in sourcing monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material solutions tailored to your assay development pipeline. Let CamelBio bridge your concept-to-clinic journey with reliable supply and expert support.