AP2B1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P63010

Core Product Specifications and Parameters

Biological Background: AP2B1 Function and Localization

• AP2B1 encodes the beta subunit of the adaptor protein complex 2 (AP-2), also known as beta-adaptin or AP105B, which is a major component of clathrin-coated vesicles.
• AP-2 functions in clathrin-dependent endocytosis by selectively sorting transmembrane cargo proteins containing YXXΦ and [ED]XXXL[LI] motifs into nascent vesicles.
• The beta subunit (AP2B1) binds directly to clathrin heavy chain to promote lattice assembly and serves as a scaffold for endocytic accessory proteins via its C‑terminal appendage domain.
• AP-2 is localized to the cell membrane and coated pits, where it coordinates cargo selection and vesicle formation.
• In hippocampal neurons, AP-2 mediates the endocytosis of ADAM10 during long-term potentiation. Related references: PMID:23676497
• AP2B1 is subject to post‑translational modifications including acetylation and phosphorylation, and is expressed in the brain at the protein level.
• Alternative splicing of AP2B1 produces multiple isoforms, potentially contributing to functional diversity in different tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 752–951 of human AP2B1, which maps to the C‑terminal appendage domain involved in protein–protein interactions; this region is relatively conserved across species, supporting cross‑reactivity with mouse and rat.
• For Western blotting, the predicted band size is approximately 105 kDa; consider using appropriate gel percentages and transfer conditions for high‑molecular‑weight proteins.
• In immunohistochemistry (paraffin‑embedded sections), target expression in brain tissue or cell lines with active endocytosis may yield strong signal; validate in the relevant sample system.
• ELISA performance may be optimized by using recombinant protein or peptide antigens corresponding to the immunogen region; confirm with positive and negative controls.
• Given its role in synaptic function, AP2B1 may be examined in neuroscience research; confirm expression and localization in your model system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a dedicated one‑stop partner for IVD raw materials, providing validated antibodies, bulk reagents, and customized solutions for diagnostic development. This anti‑AP2B1 polyclonal antibody supports research in endocytosis and neurology, and we can assist with sourcing optimized antibody pairs, ancillary reagents, or hard‑to‑find raw materials to accelerate your project from concept to clinic.