AP2B1 Rabbit Monoclonal Antibody for WB and ELISA - P63010

Core Product Specifications and Parameters

Biological Background: AP2B1 Function and Localization

• AP-2 complex subunit beta, also known as beta-2-adaptin, is a component of the heterotetrameric adaptor protein complex AP-2.
• AP-2 plays a key role in clathrin-mediated endocytosis, selecting cargo and assembling clathrin-coated vesicles at the plasma membrane.
• AP-2 recognizes specific sorting motifs (YXXΦ and [ED]XXXL[LI]) on transmembrane cargo proteins to direct their internalization.
• In hippocampal neurons, AP-2 mediates activity-dependent endocytosis of ADAM10 during long-term potentiation. Related references: PMID:23676497
• Localizes to the cell membrane, specifically clathrin-coated pits.
• Expressed in the brain at the protein level.
• Subject to acetylation and phosphorylation; keywords include alternative splicing, endocytosis, protein transport, and reference proteome.
• The canonical protein has a calculated mass of ~104.5 kDa, with observed forms at 98, 104, and 105 kDa likely reflecting isoforms or modifications.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate bands around 98–105 kDa and validate with positive control lysates such as HeLa cells or brain tissue extracts.
• Since the immunogen covers the C‑terminal appendage domain (aa 752–951), confirm that epitope accessibility is maintained under your experimental conditions.
• For ELISA applications, consider using recombinant full‑length or domain‑specific AP2B1 protein as a coating antigen or standard.
• When testing cross‑reactivity with mouse and rat samples, perform sequence alignment of the epitope region to assess potential binding differences.
• As a rabbit monoclonal antibody, optimize blocking and dilution parameters to reduce background, especially for multi‑species tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostics manufacturers, research laboratories, and life science institutions with one‑stop access to IVD raw materials, technical services, and consulting — from initial development to clinical readiness. For AP2B1 and related endocytosis or neuroscience research targets, we can support your workflow with validated monoclonal antibodies, custom recombinant proteins, and bulk ancillary reagents. Our sourcing capabilities help you secure rare‑target raw materials efficiently, enabling robust assay development and reliable supply chains.