AOC1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P19801

Core Product Specifications and Parameters

Biological Background: AOC1 Function and Localization

• AOC1 encodes diamine oxidase (DAO), a copper-containing amine oxidase also known as histaminase, amiloride-binding protein 1 (ABP1), and kidney amine oxidase (KAO).
• It catalyzes the oxidative deamination of primary amines to aldehydes, producing hydrogen peroxide and ammonia; this activity is central to histamine metabolism and polyamine catabolism. Related references: PMID:12072962, PMID:19764817, PMID:239684
• The enzyme preferentially oxidizes histamine and 1-methylhistamine, linking it to allergic and immune response regulation, and can also act on cadaverine, putrescine, spermidine, and spermine.
• Subcellular localization: AOC1 is secreted into the extracellular space and associates with the cell membrane, consistent with its role in extracellular amine clearance.
• Tissue specificity: Widely expressed with higher levels in placenta and kidney, reflecting its importance in local histamine degradation.
• Post-translational modifications include glycosylation, disulfide bond formation, and the generation of a copper-bound TPQ cofactor, which are essential for enzymatic activity.
• Alternative splicing produces multiple transcript variants, potentially influencing enzyme localization or substrate affinity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 20–300) of human AOC1; verify reactivity in your target species or sample type before large-scale experiments.
• For Western blot (WB), a band near 85 kDa is expected under reducing conditions; include appropriate loading controls and validate molecular weight markers.
• In immunofluorescence (IF/ICC), test the antibody on known AOC1-expressing cells (e.g., placental or kidney cell lines) with appropriate fixation and permeabilization protocols.
• For ELISA, consider pairing with a detection antibody against a different epitope to improve specificity; optimize coating antigen and blocking conditions.
• Cross-reactivity with mouse and rat enables comparative studies across species; however, perform a species-specific validation when switching models.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines the sourcing of critical reagents such as anti-AOC1 antibodies for allergy, immune response, and amine metabolism research. We offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, ancillary reagents, and rare-target sourcing—empowering diagnostic manufacturers and labs to accelerate development from concept to clinic.