ANTXR1 Rabbit pAb for WB and ELISA - Q9H6X2

Core Product Specifications and Parameters

Biological Background: ANTXR1 Function and Localization

• ANTXR1 (Anthrax toxin receptor 1), also known as tumor endothelial marker 8 (TEM8), is a single-pass transmembrane glycoprotein that functions as a receptor for anthrax protective antigen and mediates cell attachment and migration.
• It plays a key role in extracellular matrix (ECM) homeostasis by mediating cell adhesion to type 1 collagen and gelatin, promoting actin cytoskeleton reorganization and cell spreading.
• Localized at the cell membrane, it is enriched in lamellipodia and filopodia, suggesting involvement in cell protrusion and migration dynamics.
• ANTXR1 is highly expressed in tumor endothelial cells and has been implicated in angiogenesis, as demonstrated in cultured umbilical vein endothelial cells.
• It acts as a receptor for urokinase-type plasminogen activator (PLAU), triggering phosphorylation of EGFR and ERK1/2, which links ECM remodeling to intracellular signaling (PubMed:30241478). Related references: PMID:30241478
• Additionally, ANTXR1 serves as a cellular entry receptor for Seneca Valley virus (SVV) when glycosylated, highlighting its role in viral pathogenesis.
• Post-translational modification keywords include glycoprotein, phosphoprotein, and disulfide bond, indicating regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 84–333 of the extracellular domain, covering the von Willebrand factor type A (vWA) domain involved in ligand binding; this region may help detect both full-length and shed forms under reducing conditions.
• As a polyclonal antibody from rabbit, it may exhibit batch-dependent avidity; consider pre-adsorption or blocking steps to minimize background in highly glycosylated samples.
• For Western blot, a whole-cell lysate from endothelial (e.g., HUVEC) or tumor cell lines expressing ANTXR1 is recommended; validate bands using siRNA knockdown or a known positive control.
• In ELISA, this antibody can be used as a capture or detection reagent, but optimal coating and dilutions must be determined experimentally for each assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement hub for IVD raw materials, offering reliable access to research-grade antibodies, including this ANTXR1 rabbit polyclonal antibody. Whether you need validated antibody pairs, bulk reagents, or support for rare target sourcing, our streamlined supply network helps diagnostic manufacturers and researchers accelerate assay development. Explore our portfolio for tumor angiogenesis and ECM-related targets today.