Anti-ZZZ3 Rabbit Polyclonal Antibody for WB, ELISA - Q8IYH5

Core Product Specifications and Parameters

Biological Background: ZZZ3 Function and Localization

• ZZZ3 (ATAC1) functions as a histone H3 reader, critical for the ATAC complex-mediated maintenance of histone acetylation and gene activation. Related references: PMID:30217978
• It is an integral component of the ATAC complex, which possesses histone acetyltransferase activity targeting histones H3 and H4. Related references: PMID:19103755
• ZZZ3 features a ZZ-type zinc finger domain, facilitating DNA-binding and metal-ion coordination.
• The protein localizes to the nucleus, aligning with its role in chromatin modification and transcription regulation.
• ZZZ3 undergoes multiple post-translational modifications, including acetylation, phosphorylation, and isopeptide bond formation (ubiquitin-like conjugation), potentially influencing its activity and stability.
• Alternative splicing generates several isoforms, hinting at functional versatility.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-280), which includes the ZZ-type zinc finger domain; consider this for epitope mapping and target recognition.
• For Western blot, the predicted molecular weight is approximately 102 kDa; validate with appropriate positive control cell lysates (e.g., HeLa, HEK293) and optimize blocking and antibody incubation conditions.
• ELISA can be employed for quantitative detection; titrate the coating antigen and antibody dilution for optimal signal-to-noise ratio.
• Reported cross-reactivity with human and rat; if using in other species, verify reactivity experimentally due to sequence divergence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, streamlining your transition from concept to clinic. This ZZZ3 polyclonal antibody supports research into chromatin biology and transcriptional regulation. We supply validated antibodies, recombinant proteins, and ancillary reagents, covering rare and common targets to accelerate your diagnostic assay development.