Anti-Zyxin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15942

Core Product Specifications and Parameters

Biological Background: Zyxin Function and Localization

• Zyxin, also known as Zyxin-2, is an adhesion plaque protein encoded by the ZYX gene in humans.
• It binds alpha-actinin and the CRP protein, playing a critical role in targeting TES and ENA/VASP family members to focal adhesions.
• Zyxin contributes to the formation of actin-rich structures and may be part of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
• The protein localizes to the cytoplasm, cytoskeleton, nucleus, and specifically to cell junctions and focal adhesions.
• It contains LIM domains and zinc-binding motifs, and is subject to post-translational modifications including acetylation, phosphorylation, and methylation.
• Zyxin has been identified in proteomics studies and is involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 350-572) of human Zyxin, which includes LIM domains; consider this target region when designing complementary detection antibodies for sandwich assays.
• For Western blotting, validate in the relevant sample system; a specific band around 61 kDa is expected.
• For immunofluorescence (IF/ICC), confirm localization to focal adhesions and the actin cytoskeleton using appropriate markers.
• For IHC-P, consider optimizing antigen retrieval conditions and validate with positive and negative tissue controls.
• Due to reactivity with human, mouse, and rat, this antibody can be used in comparative studies across these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating Zyxin and its role in focal adhesion dynamics and cytoskeletal organization, we supply validated antibodies, recombinant proteins, and ancillary reagents. Our global sourcing network ensures reliable procurement of rare targets and supports bulk-scale production to accelerate your diagnostic development.