Anti-Zyxin Monoclonal Antibody for WB, IF/ICC, ELISA - Q15942

Core Product Specifications and Parameters

Biological Background: Zyxin Function and Localization

• Zyxin is an adhesion plaque protein that binds α-actinin and the CRP protein, playing a critical role in focal adhesion assembly and actin cytoskeleton organization.
• It facilitates the targeting of TES and ENA/VASP family members to focal adhesions, promoting the formation of actin-rich structures.
• Zyxin may function as a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytoskeleton, nucleus, and cell junctions, particularly focal adhesions.
• Zyxin undergoes various post-translational modifications, including acetylation, methylation, and phosphorylation, which may regulate its activity and interactions.
• It contains LIM domains and zinc-binding motifs, classifying it as a metal-binding protein with repeat regions.
• Alternative splicing produces different isoforms, and the protein has been identified through proteomics and direct protein sequencing.

Experimental Guidance and Technical Tips

• For Western blotting, consider validating the antibody's specificity by using lysates from human and mouse cell lines, as the antibody is raised against a conserved region.
• In immunofluorescence/ICC, fixation and permeabilization conditions should be optimized to detect Zyxin at focal adhesions; expect punctate staining at cell edges.
• For ELISA, the peptide immunogen can be used as a positive control to assess antibody binding.
• When using with mouse samples, verify cross-reactivity, as reactivity is reported for both human and mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For researchers focusing on cell adhesion and cytoskeletal dynamics, this Zyxin monoclonal antibody is part of our extensive catalog of validated antibodies and ancillary reagents. Whether you require bulk monoclonal antibody production, custom antibody development, or rare target sourcing, CamelBio supports your IVD projects from concept to clinic.