Anti-ZSWIM1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BR11

Core Product Specifications and Parameters

Biological Background: ZSWIM1 Function and Localization

• ZSWIM1 (Zinc finger SWIM domain-containing protein 1) is a 55 kDa polypeptide encoded by the ZSWIM1 gene (previously C20orf162) on human chromosome 20, belonging to the SWIM domain family of zinc-finger proteins.
• The SWIM domain is a conserved zinc-binding motif that coordinates one or more zinc ions, typically involved in protein-protein interactions and found in diverse eukaryotic proteins.
• UniProt classifies ZSWIM1 under keywords 'Metal-binding', 'Zinc', and 'Zinc-finger', confirming its role as a zinc-dependent regulatory factor.
• Identified at the protein level through proteomics, ZSWIM1 is part of the human reference proteome, indicating widespread tissue expression and functional relevance.
• No specific subcellular localization or tissue enrichment data are available; however, zinc-finger proteins often shuttle between nucleus and cytoplasm depending on post-translational modifications and interacting partners.
• The recombinant immunogen corresponds to the C-terminal region (amino acids 411–485), which may contain critical structural elements or interaction interfaces of the mature protein.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the C-terminal domain (aa 411–485). This segment is likely structured and accessible; consider using the full-length recombinant protein as a positive control in ELISA and Western blot.
• For Western blot: run a positive control lysate from cells expressing ZSWIM1 (e.g., HeLa, HEK293) alongside the recombinant immunogen. A band near 55 kDa is expected; minor bands may arise from polyclonal reactivity.
• For ELISA: the recombinant immunogen can serve as the coating antigen. Titrate the antibody accordingly and include appropriate negative controls.
• Cross-reactivity with mouse is reported; validate in mouse tissue or cell lines to confirm specificity before use in comparative studies.
• This polyclonal antibody may recognize multiple epitopes, increasing sensitivity but also raising background; optimize blocking and washing conditions for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering one-stop access from concept to clinic. This anti-ZSWIM1 antibody fits into research areas focused on zinc-finger proteins and their regulatory networks. Beyond single antibodies, we can source validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and even rare-target raw materials tailored to diagnostic development. Contact our team to discuss your sourcing needs and accelerate your project.