Anti-ZSCAN21 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y5A6

Core Product Specifications and Parameters

Biological Background: ZSCAN21 Function and Localization

• ZSCAN21 (Zinc finger and SCAN domain-containing protein 21), also known as ZNF38, Zipro1, and renal carcinoma antigen NY-REN-21, is a member of the SCAN domain family of transcription factors.
• It functions as a strong transcriptional activator, binding DNA via its zinc finger domains to regulate target gene expression.
• ZSCAN21 plays a critical role in spermatogenesis, where it is essential for the progression of meiotic prophase I in spermatocytes.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Structurally, ZSCAN21 contains both a SCAN domain and C2H2-type zinc finger motifs, facilitating protein-protein interactions and DNA binding.
• Post-translational modifications include ubiquitination (isopeptide bond) and possibly others as indicated by the "Ubl conjugation" keyword.
• Associated biological processes include differentiation, meiosis, transcription, and spermatogenesis, highlighting its importance in reproductive biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 130–280 of human ZSCAN21; while cross-reactivity with mouse and rat is indicated, sequence alignment in this region should be verified to ensure epitope conservation.
• For Western blotting, consider using reducing SDS-PAGE conditions and loading protein extracts from testis tissue or ZSCAN21-expressing cell lines; validate specificity via siRNA knockdown or CRISPR knockout controls.
• In ELISA, optimize coating antigen concentration and antibody dilution using recombinant ZSCAN21 protein or peptide; include appropriate negative controls to assess background binding.
• Due to its polyclonal nature, the antibody may recognize multiple epitopes; confirm the expected band size (~54 kDa) and be alert to possible additional bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material sourcing, including high-quality antibodies like this anti-ZSCAN21 polyclonal antibody. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development. From concept to clinic, CamelBio offers a streamlined procurement bridge for transcription and reproductive biology research needs.