Anti-ZSCAN1 Polyclonal Antibody for WB and ELISA - Q8NBB4

Core Product Specifications and Parameters

Biological Background: ZSCAN1 Function and Localization

• ZSCAN1 (Zinc finger and SCAN domain-containing protein 1, also known as MZF-1 or ZNF915) is a member of the SCAN domain-containing zinc finger transcription factor family.
• It contains a SCAN domain (also known as a leucine-rich domain) mediating protein-protein interactions, and multiple C2H2-type zinc finger motifs that likely bind DNA.
• The protein is predicted to function as a transcription regulator, possibly involved in controlling gene expression.
• Subcellular localization is nuclear, consistent with its role in transcriptional regulation.
• ZSCAN1 may undergo alternative splicing, producing multiple isoforms (as indicated by keywords).
• The zinc finger motifs enable binding to zinc ions, essential for structural integrity and DNA binding.
• No tissue-specific expression details are provided, but proteomics identification suggests expression in human tissues.
• Further studies are needed to elucidate its target genes and biological roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (amino acids 300-400), so the antibody may detect both full-length and potential isoforms. Validate the observed band size against the predicted molecular weight (~45 kDa) in your sample system.
• For western blot, consider using nuclear extracts or whole-cell lysates from human cell lines, as ZSCAN1 is a nuclear protein.
• In ELISA applications, the peptide immunogen can be used as a positive control for coating or competition assays.
• Optimize antibody dilution and incubation conditions empirically; recommended starting dilutions for polyclonal antibodies in WB often range from 1:500 to 1:2000, but adjust based on your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with a comprehensive one-stop supply of IVD raw materials, including validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents. For targets like ZSCAN1, a zinc finger transcription factor with emerging relevance in gene regulation studies, CamelBio offers reliable polyclonal antibodies and can assist in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.