Anti-ZPBP Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9BS86

Core Product Specifications and Parameters

Biological Background: ZPBP Function and Localization

• ZPBP, also known as ZPBP1 or Sp38, is a zona pellucida-binding protein encoded by the ZPBP gene in humans.
• It plays a role in acrosome compaction and sperm morphogenesis (PubMed:21911476).
  Related references:
  PMID:21911476
• ZPBP is implicated in sperm-oocyte interaction during fertilization (By similarity).
• The protein is located in the acrosome, a specialized secretory vesicle, and is associated with the acrosomal membrane; it is also secreted.
• ZPBP is specifically expressed in the testis.
• Post-translational modifications include glycosylation, and the protein undergoes alternative splicing.
• The molecular weight is approximately 40 kDa (calculated 40,142 Da from UniProt).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 45–200 of human ZPBP, which may target a conserved region; consider verifying cross-reactivity in mouse and rat samples.
• For Western blot (WB), a band around 40 kDa is expected; validate in testis lysates where ZPBP is specifically expressed.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), signal should localize to the acrosome of sperm cells; include appropriate controls.
• For ELISA, optimize coating antigen and antibody concentrations as per standard protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable, one-stop supply channel for critical IVD raw materials. This anti-ZPBP polyclonal antibody supports studies in reproductive biology and male infertility diagnostics. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for hard-to-find targets to accelerate your assay development from concept to clinic.