Anti-ZP3 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P21754

Core Product Specifications and Parameters

Biological Background: ZP3 Function and Localization

• Zona pellucida sperm-binding protein 3 (ZP3, also known as ZPC, ZP3A/ZP3B, Zp-3) is a major glycoprotein component of the zona pellucida, an extracellular matrix surrounding oocytes.
• ZP3 plays a critical role in fertilization: it mediates species-specific sperm binding to the oocyte, triggers the acrosome reaction, and helps prevent polyspermy after fertilization.
• The zona pellucida is typically composed of 3 to 4 glycoproteins (ZP1, ZP2, ZP3, ZP4), with ZP3 being essential for both sperm interaction and zona matrix assembly.
• ZP3 is primarily localized to the zona pellucida matrix and is also associated with the oocyte cell membrane.
• The protein undergoes post-translational modifications including glycosylation, disulfide bond formation, and cleavage at basic residues, which are important for its structural and functional roles.
• Tissue expression is restricted to oocytes, as confirmed at the protein level.
• Alternative splicing produces multiple isoforms (ZP3A and ZP3B), and mutations in ZP3 are associated with infertility and disease variants.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide derived from a sequence within the central region (aa 246-345) of human ZP3. Researchers should consider verifying the epitope specificity in their model system, particularly when studying ZP3 isoforms or cross-species reactivity.
• For Western blot (WB), a band around 47 kDa (predicted molecular weight ~47 kDa) is expected. However, due to glycosylation, the observed molecular weight may differ; consider using deglycosylation enzymes if precise molecular weight determination is required.
• In immunofluorescence (IF-P), ZP3 typically localizes to the zona pellucida and oocyte membrane; ensure proper fixation and permeabilization protocols to detect this extracellular matrix-associated protein.
• The antibody cross-reacts with human, mouse, and rat ZP3, as per supplier data, but we recommend validating in your specific tissue or cell type before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material sourcing by offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. For reproductive biology and fertility diagnostics, we provide validated anti-ZP3 antibodies along with matched pairs for assay development. Our supply capabilities include optimized monoclonal/polyclonal antibodies, bulk reagents, and support for rare target sourcing to accelerate your diagnostic pipeline.