Anti-ZP2 Polyclonal Antibody for WB - Q05996

Core Product Specifications and Parameters

Biological Background: ZP2 Function and Localization

• ZP2 (Zona pellucida sperm-binding protein 2), also known as zona pellucida glycoprotein 2 (Zp-2) and zona pellucida protein A, is an essential component of the oocyte extracellular matrix.
• As a structural component of the zona pellucida, ZP2 mediates sperm binding, triggers the acrosome reaction, and prevents post-fertilization polyspermy. It functions as a secondary sperm receptor. Related references: PMID:29895852
• The zona pellucida matrix is composed of 3 to 4 glycoproteins, including ZP1, ZP2, ZP3, and ZP4. Related references: PMID:29895852
• Subcellular localization: ZP2 is found in the zona pellucida surrounding the oocyte and is also associated with the cell membrane.
• Expression: ZP2 is specifically expressed in oocytes, as detected at the protein level.
• Post-translational modifications: ZP2 is a glycoprotein and contains disulfide bonds, which are important for its structural integrity.
• Alternative splicing: Multiple isoforms of ZP2 have been identified, suggesting regulatory diversity.

Experimental Guidance and Technical Tips

• The immunogen (aa 651-745) represents the C-terminal region of human ZP2. When using this polyclonal antibody in Western blotting, a band around 82 kDa (predicted molecular weight) may be detected in lysates from human, mouse, or rat oocytes. However, cross-species reactivity should be validated individually.
• For immunofluorescence (IF-P), consider testing on ovarian tissue sections or isolated oocytes. Optimization of fixation and permeabilization conditions is recommended.
• ELISA applications may be useful for detecting ZP2 in solution; include appropriate controls to ensure specificity.
• As a polyclonal antibody, lot-to-lot consistency may vary. It is advisable to titrate the antibody for each new batch and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw material partner, providing diagnostic manufacturers, labs, and research institutes with seamless access to a broad portfolio of antibodies and ancillary reagents. This anti-ZP2 polyclonal antibody complements our collection of validated tools for reproductive biology and infertility research. In addition to off-the-shelf products, CamelBio facilitates custom antibody development, bulk ordering, and rare target sourcing to support every step from concept to clinic.