Anti-ZP1 Rabbit Polyclonal Antibody for WB and ELISA - P60852

Core Product Specifications and Parameters

Biological Background: ZP1 Function and Localization

• ZP1, also known as zona pellucida glycoprotein 1 (Zp-1), is a zona pellucida sperm-binding protein encoded by the ZP1 gene.
• It is a key component of the zona pellucida, an extracellular matrix surrounding oocytes that mediates sperm binding, induces the acrosome reaction, and prevents post-fertilization polyspermy.
• The zona pellucida consists of three to four glycoproteins: ZP1, ZP2, ZP3, and ZP4, with ZP1 serving a critical role in maintaining the structural integrity of the matrix.
• ZP1 localizes to the zona pellucida and cell membrane, and its expression is highly specific to oocytes at the protein level.
• Post-translational modifications, including cleavage on a pair of basic residues and disulfide bond formation, are integral to ZP1's functional conformation.
• The protein has a molecular weight of approximately 70 kDa, consistent with its role as a structural glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein spanning amino acids 340–610 of human ZP1; consider verifying epitope recognition in your target application.
• For Western blot (WB) detection, prepare protein lysates from oocyte-containing tissues or relevant cell lines expressing ZP1, and optimize antibody dilution accordingly.
• In ELISA assays, validate the antibody against recombinant ZP1 protein or native zona pellucida antigens to confirm specificity and sensitivity.
• As a polyclonal antibody, it may detect multiple epitopes; always include appropriate controls to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, offering this anti-ZP1 polyclonal antibody alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need rare target antibodies for fertility research or large-scale production support, CamelBio bridges the gap from concept to clinic with reliable supply and technical expertise.