Anti-ZO-1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q07157

Core Product Specifications and Parameters

Biological Background: ZO-1 Function and Localization

• ZO-1 (Tight junction protein 1) is a scaffolding protein belonging to the TJP family, which includes TJP2 and TJP3, that links tight junction transmembrane proteins (claudins, junctional adhesion molecules, occludin) to the actin cytoskeleton.
  Related references: PMID:7798316 PMID:9792688
• The protein forms multistranded condensates that elongate into the tight junction belt, anchored apically via PATJ interaction, essential for epithelial polarization, barrier formation, and lumenogenesis.
• ZO-1 plays a regulatory role in cell migration by targeting CDC42BPB to the leading edge, and is important for podosome formation, regulating cell adhesion and matrix remodeling.
  Related references: PMID:21240187 PMID:20930113
• Together with TJP2 and TJP3, it participates in junctional retention and stability of the transcription factor DBPA, although it is not involved in its nuclear shuttling.
• ZO-1 localizes to cell membranes at tight junctions, gap junctions, and podosomes (cell projections).
• Tissue-specific expression: the alpha-containing isoform is found in most epithelial junctions, while the short isoform is detected in endothelial cells and specialized epithelial junctions of renal glomeruli and Sertoli cells.
• ZO-1 contains multiple protein interaction domains, including PDZ, SH3, and guanylate kinase-like domains, and is regulated by phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 18–273 of human ZO-1 (N-terminal region, spanning PDZ domains). Consider validating specificity with knockout or knockdown controls.
• For Western blot, expect a band at ~195 kDa. Use an appropriate gel percentage (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• In immunofluorescence on paraffin-embedded sections (IF-P), optimize antigen retrieval; verify localization at tight junctions or membrane regions.
• This antibody cross-reacts with mouse and rat; suitable for rodent model studies; however, confirm signal in the relevant tissue before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, to support tight junction research and diagnostic development. Our one-stop supply chain services enable rapid access to high-quality reagents, from concept to clinic, for manufacturers and labs worldwide.