Anti-ZO-1 Rabbit Monoclonal Antibody for IF/ICC, IHC-P, ELISA - Q07157

Core Product Specifications and Parameters

Biological Background: ZO-1 Function and Localization

• ZO-1 (Tight junction protein 1) acts as a scaffolding protein, directly linking tight junction transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton, which is fundamental for epithelial barrier establishment and paracellular permeability control.
  Related references: PMID:7798316, PMID:9792688
• ZO-1 forms multistranded condensates that elongate into tight junction belts, anchored at the apical cell membrane via interaction with PATJ; this process is necessary for lumenogenesis, efficient epithelial polarization, and barrier formation.
• ZO-1 regulates cell migration by recruiting CDC42BPB to the leading edge of migrating cells.
  Related references: PMID:21240187
• In podosomes, ZO-1 governs cell adhesion and matrix remodeling, contributing to processes such as cell invasion and tissue remodeling.
  Related references: PMID:20930113
• The alpha-containing isoform of ZO-1 is predominantly expressed in most epithelial cell junctions, whereas a shorter isoform is found in endothelial cells and specialized epithelial junctions of renal glomeruli and Sertoli cells.
• ZO-1 localizes to cell membranes, tight junctions, gap junctions, and podosomes, and features domains such as SH3 and calmodulin-binding motifs, along with multiple phosphorylation sites, enabling dynamic regulatory interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-320 of human ZO-1) is conserved across human, mouse, and rat, likely underpinning the reported cross-reactivity; nonetheless, verify specificity in your experimental model system.
• For immunofluorescence (IF/ICC), consider using methanol or paraformaldehyde fixation with appropriate permeabilization, as tight junction staining patterns can be sensitive to these conditions.
• In IHC-paraffin (IHC-P), heat-induced antigen retrieval (e.g., citrate or EDTA buffer) may be required to expose the epitope; validate with appropriate controls.
• For ELISA, the monoclonal format allows use as a capture or detection antibody; pairing with a matched antibody may be explored for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined one-stop channel for IVD raw materials, from concept to clinic. This ZO-1 rabbit monoclonal antibody supports research into tight junctions, epithelial barrier function, and related disease models. Beyond antibodies, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your IVD development workflows.