Anti-ZNF785 Polyclonal Antibody for WB, ELISA - A8K8V0

Core Product Specifications and Parameters

Biological Background: ZNF785 Function and Localization

• ZNF785 encodes a zinc finger protein 785, which is a member of the C2H2-type zinc finger family. These proteins are characterized by multiple zinc finger domains that facilitate DNA binding.
• The protein contains several zinc finger repeats (as indicated by the UniProt keyword "Repeat"), suggesting a modular structure typical of transcription factors.
• Based on the presence of zinc finger domains and its annotation as a DNA-binding protein, ZNF785 is likely involved in transcriptional regulation, though its exact function remains to be fully elucidated.
• Consistent with its predicted role, ZNF785 is localized to the nucleus (UniProt: SUBCELLULAR LOCATION: Nucleus).
• The gene is subject to alternative splicing, potentially generating multiple protein isoforms with distinct properties.
• ZNF785 has been identified in large-scale proteomics studies (keyword: Proteomics identification), confirming its expression at the protein level in human cells.
• It is part of the UniProt Reference proteome, indicating that it is a well-supported gene model with high-quality protein sequence data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–405 of human ZNF785, a region that contains zinc finger domains. This sequence is conserved in mouse (cross-reactivity indicated), making the antibody suitable for studies in both species.
• For Western blot, load 20–30 μg of whole-cell lysate from human or mouse cell lines/tissues known to express ZNF785. The predicted molecular weight is ~46 kDa; however, alternative splicing and post-translational modifications may result in additional bands.
• As a polyclonal antibody, batch-to-batch variability can occur. It is recommended to validate binding specificity and optimal working dilution in the relevant sample system.
• For ELISA, use either direct coating with recombinant ZNF785 protein or a sandwich format with a matched antibody pair. Perform cross-titration and check background with negative controls.
• Consider pre-incubating the antibody with the immunogen or a blocking peptide to confirm specificity in both WB and ELISA.
• Since ZNF785 is a DNA-binding transcription factor, epitope accessibility may be influenced by chromatin association. Consider testing with nuclear extracts or under denaturing conditions for improved detection.

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