Anti-ZNF777 Polyclonal Antibody for WB, ELISA - Q9ULD5

Core Product Specifications and Parameters

Biological Background: ZNF777 Function and Localization

• ZNF777 acts as a transcriptional repressor, potentially involved in regulating gene expression. Related references: PMID:31856708
• It inhibits cell proliferation through induction of CDKN1A/p21 by down-regulating NIBAN1/FAM129A at low cell density. Related references: PMID:25560148
• Contains multiple zinc-finger motifs, suggesting direct DNA binding and transcriptional regulatory functions.
• Localizes predominantly to the nucleus, consistent with its role in transcription regulation.
• ZNF777 undergoes post-translational modifications including phosphorylation and ubiquitination, which may modulate its activity.
• Additional annotations: metal-binding, repeat domain structure, and involvement in transcription regulation.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~94 kDa; consider preparing lysates from human or mouse cell lines expressing ZNF777.
• As a polyclonal antibody, validate specificity by including a no-primary control and pre-immune serum if available.
• For ELISA, coat with the recombinant immunogen or a target-derived peptide to assess binding affinity; optimize coating conditions.
• Cross-reactivity with mouse allows for potential use in mouse models; verify in the relevant tissue or cell system.
• Consider using blocking buffers containing 5% non-fat milk or BSA to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibodies for transcription and cancer research. Our ZNF777 Rabbit pAb exemplifies our commitment to supplying well-characterized reagents for Western blot and ELISA applications. Beyond this product, CamelBio offers bulk ancillary reagents, customized antibody development, and rare target sourcing to support your entire workflow from concept to clinic.