Anti-ZNF768 Polyclonal Antibody for WB, ELISA - Q9H5H4

Core Product Specifications and Parameters

Biological Background: ZNF768 Function and Localization

• ZNF768 (Zinc finger protein 768) is a DNA-binding protein that specifically recognizes mammalian-wide interspersed repeats (MIRs) in euchromatin and gene promoter regions via a consensus bipartite sequence 5'-GCTGTGTG-[N20]-CCTCTCTG-3'. Related references: PMID:30476274

• It is required for cell homeostasis, as shown by functional studies. Related references: PMID:34404770

• ZNF768 likely participates in transcriptional regulation, though its precise role remains to be fully elucidated.

• The protein localizes to the nucleus and associates with chromosomes, consistent with its chromatin-binding activity.

• ZNF768 is a member of the zinc finger protein family, characterized by C2H2-type zinc finger domains that mediate DNA binding and may also facilitate protein-protein interactions.

• It is classified as a phosphoprotein and contains metal-binding zinc ions; these post-translational modifications may regulate its activity or stability.

• Keywords from UniProt include: Chromosome, DNA-binding, Metal-binding, Nucleus, Phosphoprotein, Repeat, Transcription, Transcription regulation, Zinc, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 140–270 of human ZNF768, a region that may encompass one or more zinc finger domains. Researchers should consider that this region is likely to be structurally stable and exposed in the native protein, making it a suitable antigen for generating antibodies that recognize both denatured (WB) and native (ELISA) forms.
• For Western blot, a band at approximately 60 kDa is expected. Validate antibody specificity using positive controls expressing ZNF768 and negative controls such as knockout lysates or tissues with low expression.
• When performing ELISA, titrate the antibody and consider using recombinant ZNF768 protein as a standard to ensure assay sensitivity and linearity.
• The antibody cross-reacts with mouse ZNF768, enabling studies in mouse models. However, confirm cross-reactivity in the specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers and researchers with a streamlined pathway to high-quality reagents. For transcription and chromatin biology applications, CamelBio offers this anti-ZNF768 polyclonal antibody, along with validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and customized sourcing for difficult-to-find targets. Leverage our supply capabilities to accelerate your assay development from concept to clinic.