Anti-ZNF76 Rabbit Polyclonal Antibody for WB, ELISA - P36508

Core Product Specifications and Parameters

Biological Background: ZNF76 Function and Localization

• ZNF76, also known as zinc finger protein 523 (ZNF523), is a transcription factor that belongs to the C2H2-type zinc finger protein family.

• ZNF76 regulates genes transcribed by both RNA polymerase II and III, recognizing the Staf-binding site (SBS) consensus motif in target promoters. Related references: PMID:10893243 PMID:9705341

• It activates small nuclear RNA (snRNA) gene expression and can function as a DNA-binding transcriptional repressor by inhibiting TATA-binding protein (TBP) activity. Related references: PMID:15280358 PMID:16337145

• ZNF76 localizes to the nucleus and associates with chromosomes, consistent with its role in transcriptional regulation.

• The protein is widely expressed across tissues, with particularly high expression observed in testis.

• Key structural features include multiple zinc finger domains that mediate DNA binding, and the protein is subject to alternative splicing and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region containing zinc finger motifs (amino acids 100–170), suggesting the antibody may recognize epitopes within the DNA-binding domain.
• For Western blot, the predicted molecular weight of ZNF76 is approximately 62 kDa; consider using a gel percentage suitable for this size range.
• Given its polyclonal nature, the antibody likely detects multiple epitopes, which may enhance sensitivity but also increases the importance of proper blocking and negative controls.
• Cross-reactivity with mouse and rat is predicted; validate the antibody in the relevant sample system if using these species.
• For ELISA, the antibody may be used to capture or detect recombinant or native ZNF76; titration and buffer optimization are recommended.

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