Anti-ZNF76 Polyclonal Antibody for WB and ELISA - P36508

Core Product Specifications and Parameters

Biological Background: ZNF76 Function and Localization

• ZNF76 (Zinc finger protein 76), also known as ZNF523, is a zinc-finger transcription factor that regulates genes transcribed by RNA polymerases II and III.
  Related references:
  PMID:10893243
  PMID:9705341
• It specifically recognizes the Staf-binding site (SBS), a conserved DNA motif found in many gene promoters, and can activate expression of small nuclear RNAs (snRNAs) transcribed by both polymerases.
  Related references:
  PMID:9705341
• ZNF76 also functions as a repressor of mRNA transcription by inhibiting the activity of the TATA-binding protein (TBP).
  Related references:
  PMID:15280358
  PMID:16337145
• The protein is localized to the nucleus and associates with chromosomes, consistent with its role in transcription regulation.
• ZNF76 undergoes alternative splicing, producing isoforms with predicted molecular weights around 56 kDa and 61 kDa, matching the observed doublet in some systems.
• It contains multiple C2H2-type zinc finger motifs for DNA binding and harbors isopeptide bonds and ubiquitin-like conjugation, suggesting post-translational regulatory modifications.
• Widely expressed in tissues, with notably high expression in testis, indicating potential roles in gametogenesis or tissue-specific transcription programs.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 463–570 near the C-terminal region, which may allow detection of both major isoforms; however, confirm isoform specificity by molecular weight shift or isoform-specific controls.
• For Western blot, use reducing conditions and load adequate protein from testis or other ZNF76-expressing cell lines; expect bands around 56–61 kDa.
• When applying in ELISA, consider using recombinant ZNF76 fragments or cell lysates, and validate the antibody’s reactivity under your coating/detection conditions.
• Cross-reactivity with mouse and rat has been indicated; however, always sequence align the target epitope across species to ensure consistent performance in non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw-material sourcing, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom procurement of rare target materials. This anti-ZNF76 antibody supports researchers studying transcription regulation and chromatin biology. Contact our team to accelerate your diagnostic development from concept to clinic with comprehensive supply and technical support.