Anti-ZNF683 Rabbit Polyclonal Antibody for WB, ELISA - Q8IZ20

Core Product Specifications and Parameters

Biological Background: ZNF683 Function and Localization

• ZNF683 (Hobit) is a zinc-finger transcription factor essential for the development and retention of tissue-resident memory T (Trm) cells, natural killer (NK) cells, and natural killer T (NKT) cells in non-lymphoid organs such as skin, gut, liver, and kidney. Related references: PMID:26179882
• It functions by binding to promoter regions of target genes, often in association with the transcriptional repressor PRDM1/BLIMP1, to negatively regulate genes that promote lymphocyte egress from tissues. Related references: PMID:26179882
• Isoform-specific activities have been reported: isoform 2 functions as a transcriptional repressor regulating interferon-gamma (IFN-γ) production, while isoform 1 lacks repressor activity but retains DNA-binding capability.
• ZNF683 is predominantly expressed in terminally differentiated effector CD8+ T-cells, NK cells, and NKT cells, with distinct isoform expression patterns. Related references: PMID:26179882, PMID:20921622
• During cytomegalovirus (CMV) infection, ZNF683 is upregulated in effector CD8+ T-cells, suggesting a role in antiviral immune responses. Related references: PMID:20921622
• Localized to the nucleus, ZNF683 belongs to the krueppel C2H2-type zinc-finger protein family and contains metal-binding zinc finger domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 420-524) and may recognize multiple ZNF683 isoforms; validate specificity in your experimental system.
• For Western blot, the predicted molecular weight is approximately 57 kDa; verify band migration using appropriate positive controls.
• Consider testing the antibody on human lymphocyte subpopulations or cell lines known to express ZNF683.
• The antibody is suitable for WB and ELISA as indicated; optimize dilutions and conditions for other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, including validated polyclonal antibodies like this anti-ZNF683 antibody, which is ideal for research on tissue-resident lymphocytes and immune cell retention. From optimized antibody pairs and ancillary reagents to rare target sourcing, CamelBio supports your transition from concept to clinic with tailored procurement solutions.