Anti-ZNF677 Rabbit Polyclonal Antibody for WB, ELISA - Q86XU0

Core Product Specifications and Parameters

Biological Background: ZNF677 Function and Localization

• ZNF677 (Zinc finger protein 677) is a nuclear protein that may function as a transcriptional regulator.
• It contains multiple C2H2-type zinc finger motifs, which are characteristic of DNA-binding proteins and often mediate sequence-specific interactions with nucleic acids.
• Zinc finger proteins like ZNF677 play critical roles in gene expression, chromatin remodeling, and other nuclear processes.
• Subcellular localization to the nucleus supports its proposed role in transcriptional regulation.
• ZNF677 belongs to the large Krüppel-associated box (KRAB) zinc finger protein family, although it lacks a KRAB domain; its non-KRAB zinc fingers may confer distinct regulatory functions.
• It is a member of the broader zinc finger protein superfamily, which participates in development, differentiation, and disease.
• The protein is encoded by the ZNF677 gene in humans, and its conserved zinc finger structure implies evolutionary importance in metazoan biology.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider the predicted molecular weight of approximately 68 kDa and include appropriate pre-stained markers for size confirmation.
• Validate the antibody in your specific sample system, as cross-reactivity with mouse and rat is predicted but should be experimentally confirmed.
• For ELISA, titrate the antibody and optimize coating antigen concentration to achieve the best signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot variability is possible; it is advisable to confirm performance with positive and negative controls in preliminary experiments.
• The immunogen spans amino acids 50–200, a region likely accessible for antibody binding; however, ensure that the target protein conformation (native vs. denatured) matches your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials, technical services, and expert consulting. With this ZNF677 polyclonal antibody, we help advance studies in transcriptional regulation and DNA-binding protein research. As your dedicated sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal options, bulk ancillary reagents, and rare target raw-material procurement—empowering you to streamline assay development and accelerate your path to the clinic.