Anti-ZNF677 Rabbit Polyclonal Antibody for WB and ELISA - Q86XU0

Core Product Specifications and Parameters

Biological Background: ZNF677 Function and Localization

• ZNF677 (Zinc finger protein 677) is a member of the krüppel C2H2-type zinc-finger protein family, predicted to function as a DNA-binding transcription factor.
• It contains multiple C2H2 zinc-finger domains, which are typically involved in sequence-specific DNA binding and protein-protein interactions.
• The protein is localized to the nucleus, consistent with a role in transcriptional regulation.
• Functional annotation suggests ZNF677 may be involved in transcriptional regulation, though specific target genes remain to be characterized.
• UniProt keywords classify ZNF677 as a DNA-binding, nuclear protein with zinc-finger repeats, highlighting its putative role in gene expression control.
• The predicted molecular weight of ZNF677 is approximately 68 kDa, with no known isoforms reported.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 50-200 of human ZNF677. For Western blot, consider using nuclear extract preparations to enrich the target protein.
• Validate antibody specificity by testing in ZNF677-overexpressing cell lines or using siRNA knockdown controls.
• For ELISA, coat the plate with the recombinant immunogen or purified protein; optimize antibody dilution and detection conditions.
• The antibody shows cross-reactivity with rat ZNF677, but performance in rat samples should be independently validated under your experimental conditions.
• Since ZNF677 is a transcription factor, low endogenous expression may require sensitive detection methods; consider long exposure or signal amplification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with reliable access to high-quality raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. For transcription factor targets like ZNF677, CamelBio offers sourcing support for validated antibody pairs and bulk materials, streamlining your development from discovery to diagnostic application. Our comprehensive supply chain covers rare targets and ensures consistent lot-to-lot performance for your assay needs.