Anti-ZNF652 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y2D9

Core Product Specifications and Parameters

Biological Background: ZNF652 Function and Localization

• ZNF652, also known as zinc finger protein 652, is a DNA-binding transcription repressor that cooperates with CBFA2T2 to suppress target gene expression, such as TCF12. Related references: PMID:16966434, PMID:18456661, PMID:21678463
• ZNF652 specifically recognizes the DNA consensus motif 5'-CGAAAGGGTTAAT-3'. Related references: PMID:18456661, PMID:21678463
• ZNF652 is primarily localized in the nucleus, consistent with its role as a transcriptional regulator.
• It belongs to the zinc-finger family, containing C2H2-type zinc finger domains that mediate DNA binding.
• ZNF652 is widely expressed across tissues, with elevated levels in breast, prostate, vulva, and pancreas, suggesting tissue-specific regulatory roles.
• Post-translational modifications include phosphorylation (indicated by the Phosphoprotein keyword), which may modulate its activity.
• The gene ZNF652 (KIAA0924) encodes a protein of approximately 70 kDa (predicted molecular weight 69.7 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–74) of human ZNF652, which may provide epitopes accessible in both native and denatured conditions, suitable for WB and ELISA.
• For Western blotting, consider using nuclear extract or whole cell lysate from cell lines with confirmed ZNF652 expression (e.g., breast or prostate cancer cells) and validate the ~70 kDa band.
• For ELISA, the polyclonal nature of this antibody may offer sensitive detection of ZNF652 in various sample formats; optimization of coating antigen and detection antibodies is recommended.
• Due to cross-reactivity with mouse and rat, this antibody may be applicable in rodent model studies, but always validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and researchers, offering validated antibodies such as this anti-ZNF652 polyclonal antibody alongside optimized monoclonals, bulk ancillary reagents, and rare target sourcing. Whether you need antibody pairs for immunoassay development or consistent lot-to-lot performance in WB and ELISA, our platform bridges technical supply gaps from concept to clinic.