Anti-ZNF645 Polyclonal Antibody for WB - Q8N7E2

Core Product Specifications and Parameters

Biological Background: ZNF645 Function and Localization

• E3 ubiquitin-protein ligase CBLL2 (also known as c-Cbl-like protein 2, RING-type E3 ubiquitin transferase ZNF645, Zinc finger protein 645, and CT138/HAKAIL) is encoded by the CBLL2 (ZNF645) gene in humans.
• Catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins, functioning as an E3 ubiquitin ligase. May specifically operate on tyrosine-phosphorylated SRC substrates. Related references: PMID:20657603 PMID:22252131
• Belongs to the Cbl proto-oncogene-like protein family, sharing structural features with c-Cbl. Contains a RING-type zinc finger domain, indicative of metal-binding and transferase activity.
• Localizes to the cytoplasm.
• Exclusively expressed in testis and sperm, including spermatocytes, round and elongated spermatids, and Leydig cells.
• Involved in the ubiquitin conjugation pathway (Ubl conjugation) and is annotated with the keywords metal-binding, zinc, zinc-finger, and transferase.
• The reference proteome has a calculated molecular weight of approximately 49 kDa (UniProt: 48.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 346-425 of human ZNF645. For Western blot (WB), validate the antibody using testis or sperm lysates, where ZNF645 is exclusively expressed. Consider testing a dilution series starting at 1:500-1:2000.
• For ELISA applications, the recombinant protein fragment used for immunization may serve as a suitable positive control. Cross-reactivity with mouse and rat indicates potential utility in rodent models; however, epitope conservation should be verified.
• This polyclonal antibody recognizes native and denatured forms; consider including appropriate blocking and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This ZNF645 rabbit pAb supports research in ubiquitination pathways and testis-specific protein function, compatible with various immunoassay development needs. With end-to-end technical support and reliable supply chains, CamelBio helps diagnostic manufacturers accelerate from concept to clinic.