Anti-ZNF620 Rabbit Polyclonal Antibody for WB and ELISA - Q6ZNG0

Core Product Specifications and Parameters

Biological Background: ZNF620 Function and Localization

• ZNF620 (Zinc finger protein 620) is a member of the C2H2-type zinc finger protein family, characterized by multiple zinc finger motifs that mediate DNA binding.
• The protein is predicted to function as a transcription factor, potentially involved in transcriptional regulation (UniProt).
• It localizes to the nucleus, consistent with its role in controlling gene expression (UniProt).
• Alternative splicing of the ZNF620 gene may generate multiple protein isoforms, adding functional diversity (as indicated by the keyword "Alternative splicing").
• ZNF620 contains DNA-binding zinc finger domains, suggesting direct interaction with genomic DNA to regulate target genes.
• It has been identified in proteomics studies and is part of the human reference proteome.
• The predicted molecular weight is approximately 48.5 kDa (UniProt), consistent with the ~49 kDa band observed.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody comprises amino acids 1-150 of human ZNF620 (NP_001243096.1). Researchers are advised to consider epitope mapping if isoform-specific detection is required.
• This antibody is validated for Western blot (WB) and ELISA. For WB, optimization of primary antibody dilution and sample preparation (e.g., nuclear fraction enrichment) may be beneficial given the nuclear localization of ZNF620.
• Cross-reactivity with mouse is reported; users should verify performance in their specific mouse tissue or cell line models.
• Transcription factors like ZNF620 are often low-abundance proteins; nuclear extraction or signal amplification techniques could improve detection sensitivity.
• The expected band size is ~49 kDa; ensure adequate separation by using an appropriate SDS-PAGE gel percentage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This ZNF620 antibody is an example of our portfolio supporting transcription regulation studies; we can also help source rare targets and provide custom antibody development. Rely on CamelBio to bridge your research and diagnostic manufacturing needs with reliable, high-quality supplies.