Anti-ZNF574 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q6ZN55

Core Product Specifications and Parameters

Biological Background: ZNF574 Function and Localization

• ZNF574 is a zinc finger protein that belongs to a family of transcription factors characterized by C2H2-type zinc finger domains, implicating it in DNA binding and gene regulation.
• It functions as part of a ribosome quality control pathway that surveils ribosome assembly and promotes the degradation of defective ribosomes, maintaining cellular proteostasis.
• The protein contains multiple zinc finger motifs, coordinating zinc ions and potentially mediating interactions with nucleic acids or proteins.
• ZNF574 localizes to both the cytoplasm and the nucleus, indicating dynamic shuttling or compartment-specific roles.
• Post-translational modifications such as phosphorylation and methylation have been identified, potentially regulating its activity or interactions.
• Alternative splicing produces multiple isoforms, which may exhibit distinct functions or expression patterns.
• The full-length protein has a molecular weight of approximately 99 kDa, consistent with the size observed in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 170–310; thus, the antibody may recognize isoforms containing this region but may not detect N- or C-terminal variants.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing fixation and permeabilization protocols to balance epitope preservation and accessibility, especially given the nuclear and cytoplasmic localization.
• This polyclonal antibody is suitable for ELISA; users may consider it as a capture or detection reagent in sandwich ELISA configurations.
• Given the cross-reactivity with mouse and rat, the antibody can be used to detect ZNF574 orthologs in these species, but validation in the relevant tissue or cell type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw-material sourcing, helping diagnostic manufacturers and research laboratories efficiently access high-quality reagents. The ZNF574 antibody supports investigations into ribosome quality control and gene regulation, critical areas in cellular homeostasis research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare target raw materials to meet your project needs from concept to clinic.