Anti-ZNF530 Rabbit Polyclonal Antibody for WB, ELISA - Q6P9A1

Core Product Specifications and Parameters

Biological Background: ZNF530 Function and Localization

• ZNF530 belongs to the C2H2-type zinc finger protein family, characterized by multiple zinc finger domains that mediate DNA binding and regulatory functions.
• Encoded by the ZNF530 gene (also known as KIAA1508) in humans (Gene ID: 348327), the protein is part of a large family of transcription factors.
• Putative function as a transcriptional regulator, likely modulating gene expression through direct DNA interaction via its zinc finger motifs.
• Primarily localized to the nucleus, consistent with its predicted role in transcription and chromatin organization.
• Contains conserved cysteine and histidine residues that coordinate zinc ions, essential for structural integrity and DNA-binding activity.
• Identified in proteome-wide expression studies, suggesting a broad tissue distribution as part of the reference proteome.
• The antibody detects a band at approximately 69 kDa, matching the predicted molecular weight of ZNF530 (68.8 kDa) based on its amino acid sequence.

Experimental Guidance and Technical Tips

• For Western blot, consider testing both reducing and non-reducing conditions, as the N-terminal immunogen (aa 1–190) may expose linear epitopes sensitive to denaturation.
• In ELISA, the antibody may be suitable for direct or capture formats using recombinant ZNF530 or cell lysates; optimize coating and detection conditions for best signal-to-noise ratio.
• Cross-reactivity is reported for human, mouse, and rat; validate the antibody in the relevant species by comparing band patterns or titration curves to ensure specificity.
• Due to the polyclonal nature, lot-to-lot variation may occur; it is advisable to reserve and qualify a single lot for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents for diagnostic development. For research focused on zinc finger transcriptional regulators like ZNF530, we supply validated polyclonal and monoclonal antibodies, optimised antibody pairs, and bulk quantities to support your assay workflows. Our global sourcing capability ensures timely access to even rare targets, helping you move seamlessly from discovery to clinical application.