Anti-ZNF521 Polyclonal Antibody for WB, ELISA - Q96K83

Core Product Specifications and Parameters

Biological Background: ZNF521 Function and Localization

• ZNF521, also known as early hematopoietic zinc finger protein (EHZF) or LYST-interacting protein 3 (LIP3), is a zinc finger transcription factor that functions as both an activator and repressor depending on cellular context.
• It plays a critical role in BMP signaling by associating with SMAD proteins in response to BMP2, thereby activating transcription of BMP target genes.
• ZNF521 acts as a transcriptional repressor in B-cell lineage specification by interacting with EBF1, preventing its DNA binding and activation of target genes.
• The protein is predominantly localized to the nucleus, where it exerts its transcriptional regulatory functions.
• ZNF521 is highly expressed in hematopoietic stem and progenitor cells, particularly CD34+ cells, and its levels decline rapidly during differentiation in response to hemopoietins.
• It is detected in tissues rich in stem and progenitor cells, such as placenta, spleen, lymph nodes, thymus, bone marrow, and fetal liver, but is absent in mature peripheral blood leukocytes.
• Keywords associated with ZNF521 include developmental protein, differentiation, proto-oncogene, and chromosomal rearrangement, underscoring its role in development and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 210-420 of human ZNF521, covering a region that likely contains multiple zinc finger domains; this design may yield reactivity against both full-length and potential isoforms.
• For Western blotting, anticipate a band around 148 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In ELISA applications, validate the antibody in the relevant sample matrix to account for potential cross-reactivity with other zinc finger proteins.
• Although the antibody shows cross-reactivity with rat, confirm specificity in your experimental system, particularly if using tissues with high endogenous ZNF521 expression like bone marrow or spleen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing high-quality antibodies like this anti-ZNF521 polyclonal antibody for researchers investigating hematopoietic development and transcriptional regulation. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials, supporting every stage from concept to clinic. Whether you need custom development or reliable supply, CamelBio streamlines procurement for diagnostic and research laboratories worldwide.