Anti-ZNF471 Rabbit Polyclonal Antibody for WB, ELISA - Q9BX82

Core Product Specifications and Parameters

Biological Background: ZNF471 Function and Localization

• ZNF471, also known as ERP1 or ZNF471, is a zinc finger protein belonging to the Krüppel C2H2-type zinc-finger family. It contains multiple zinc finger domains that likely mediate DNA binding.
• The ZNF471 gene undergoes alternative splicing, potentially giving rise to multiple protein isoforms.
• ZNF471 is localized in the nucleus, consistent with its predicted role in transcriptional regulation.
• The protein may function as a transcription factor, participating in the regulation of gene expression.
• Key structural features include C2H2 zinc finger repeats, which facilitate DNA interaction and metal ion coordination (zinc).
• Proteomic studies have identified ZNF471 in reference proteome datasets, supporting its expression and relevance in human biology.
• No specific post-translational modifications or tissue-specific expression patterns have been characterized, but its DNA-binding activity suggests involvement in chromatin-related processes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 24-221, spanning the N-terminal region upstream of the zinc finger domains; this region may offer specificity advantages over full-length constructs.
• For WB, consider using whole cell lysates from human cell lines (e.g., HeLa, HEK293) as positive controls; validate with siRNA knockdown or overexpression systems.
• For ELISA, direct coating with the recombinant immunogen fragment is recommended; when developing sandwich ELISA, ensure the epitope is accessible and consider pairing with a matched detection antibody.
• Cross-reactivity: The antibody is reported to react with human ZNF471; verify cross-reactivity with orthologs or isoforms through sequence alignment and experimental testing if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription factor research, including studies on ZNF471 and related zinc finger proteins, we offer validated polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to discuss your customized procurement needs.