Anti-ZNF460 Polyclonal Antibody for WB and ELISA - Q14592

Core Product Specifications and Parameters

Biological Background: ZNF460 Function and Localization

• ZNF460 (Zinc finger protein 460) is a member of the Krüppel-type C2H2 zinc finger protein family, believed to function as a DNA-binding transcription factor.
• It contains multiple zinc finger domains that may mediate sequence-specific DNA recognition and transcriptional regulation.
• The gene undergoes alternative splicing, potentially yielding multiple protein isoforms.
• ZNF460 localizes to the nucleus, consistent with its predicted role in gene expression control.
• It is ubiquitously expressed at low levels in human tissues, with relatively higher expression observed in pancreas and liver.
• Post-translational modifications include isopeptide bond formation via ubiquitin-like conjugation (Ubl conjugation), which may modulate protein stability or activity.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to a central region (aa 100-210) of human ZNF460. The antibody is expected to recognize full-length endogenous ZNF460 and may detect isoforms containing this region.
• For Western blot, consider using nuclear extracts to enrich the target protein, given its nuclear localization.
• In ELISA, validate the coating antigen and perform cross-reactivity tests with ZNF460 isoforms or closely related zinc finger proteins to confirm specificity.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ZNF460 polyclonal antibody supports investigations into transcriptional regulation and zinc finger protein biology. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your research and diagnostic development.