Anti-ZNF451 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4E5

Core Product Specifications and Parameters

Biological Background: ZNF451 Function and Localization

• ZNF451 (Zinc finger protein 451) is also known as E3 SUMO-protein ligase ZNF451, coactivator for steroid receptors (COASTER), and ZATT. It belongs to the estrogen receptor (ER)-associated protein family that interacts with steroid receptors.
• Acts as an E3 SUMO-protein ligase with a strong preference for SUMO2 and SUMO3, facilitating UBE2I/UBC9-mediated sumoylation of target proteins. Related references: PMID:26524493, PMID:26524494
• Plays a critical role in protein SUMO2 modification under stress conditions such as DNA damage and proteasome inhibition (in vitro), and is required for MCM4 sumoylation.
• Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site Lys-11; has no activity with SUMO1. Related references: PMID:26524493
• Negatively regulates transcriptional activation by the SMAD4 complex in response to TGF-beta signaling and inhibits EP300-mediated acetylation of histone H3 at Lys-9. Related references: PMID:24324267
• Participates in regulating the transcription of androgen receptor (AR) targets. Related references: PMID:18656483
• Localizes predominantly to the nucleus, including nucleoplasm and PML bodies, consistent with its roles in transcription regulation and sumoylation.
• Keywords highlight features such as zinc-finger domains, alternative splicing, phosphorylation, methylation, and its involvement in Ubl conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 600-900) of human ZNF451, which is expected to recognize multiple isoforms due to its polyclonal nature.
• For Western blotting, a band around 121 kDa is anticipated. Consider using a gradient gel and appropriate molecular weight markers for high-molecular-weight protein transfer and detection.
• Species reactivity is confirmed for human, mouse, and rat. Cross-reactivity with other species should be verified experimentally if needed.
• In ELISA, optimize coating antigen concentration and antibody dilution using the immunogen peptide or full-length protein as a positive control.
• The antibody’s performance in immunoprecipitation or immunofluorescence has not been characterized; these applications may require further validation.
• Given ZNF451’s involvement in stress-induced sumoylation, it may be beneficial to include stress controls (e.g., DNA damage or proteasome inhibition) in functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive range of IVD raw materials, including this anti-ZNF451 polyclonal antibody. Whether you are investigating SUMOylation pathways, transcriptional regulation, or the cellular stress response, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your assay development and scale-up needs. Partner with CamelBio to bridge the gap from concept to clinic efficiently.