Anti-ZNF433 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q8N7K0

Core Product Specifications and Parameters

Biological Background: ZNF433 Function and Localization

• Zinc finger protein 433 (ZNF433) is a member of the C2H2-type zinc finger protein family, which are characterized by multiple zinc finger repeats that mediate sequence-specific DNA binding.
• ZNF433 is predicted to function as a transcription factor, potentially involved in the regulation of gene expression, although its precise transcriptional targets remain to be fully characterized.
• The protein localizes to the nucleus, consistent with its putative role in transcriptional regulation and DNA-binding activity.
• Alternative splicing of the ZNF433 gene may produce multiple isoforms, which could exhibit distinct regulatory functions or expression patterns.
• Proteomic studies have identified ZNF433 in human cell and tissue lysates, indicating its expression across various biological contexts.
• Keywords associated with ZNF433 include DNA-binding, metal-binding (zinc), transcription regulation, and nuclear localization, highlighting its classification as a transcriptional regulator.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fragment corresponding to amino acids 564–673 of human ZNF433 (NP_001073880.1), targeting the C-terminal region. Researchers may consider this when designing epitope-specific competition assays.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods such as heat-induced epitope retrieval in citrate buffer to optimize nuclear staining.
• In immunofluorescence (IF/ICC), permeabilization with a detergent such as Triton X-100 is typically required to allow antibody access to the nuclear ZNF433 protein.
• The antibody's cross-reactivity with mouse and rat ZNF433 suggests the epitope is conserved; however, validation in the specific species and tissue of interest is recommended.
• For ELISA applications, pairing with a suitable secondary antibody and optimizing coating conditions may enhance signal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic laboratories with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-ZNF433 polyclonal antibody supports research into zinc-finger-mediated transcription regulation and nuclear protein dynamics. As your sourcing partner, CamelBio offers tailored procurement solutions and technical consulting to help accelerate assay development and clinical translation.