Anti-ZNF416 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q9BWM5

Core Product Specifications and Parameters

Biological Background: ZNF416 Function and Localization

• ZNF416 is a member of the zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that mediate DNA binding.
• It functions as a mechanosensitive transcriptional activator in fibroblasts, responding to changes in extracellular matrix stiffness.
• ZNF416 regulates the expression of genes involved in cell proliferation, extracellular matrix synthesis, and contractility, thereby influencing fibroblast behavior.
• The protein is localized to the nucleus, consistent with its role in transcriptional regulation.
• As a DNA-binding transcription factor, ZNF416 contains zinc finger repeats that likely recognize specific DNA sequences to modulate target gene expression.
• Its activation is triggered by mechanical cues, linking physical microenvironment signals to gene expression changes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 495–594 of human ZNF416; consider that this region may not be present in all potential isoforms—validate detection in your specific sample context.
• For IHC-P and IF/ICC, optimize antigen retrieval, fixation, and permeabilization protocols to achieve clear nuclear staining in fibroblasts or other relevant cell types.
• Cross-reactivity with mouse and rat is indicated; verify species-specific reactivity in your assay system using appropriate controls.
• For ELISA applications, use recombinant ZNF416 protein or peptide as coating antigen and titrate antibody concentration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material partner, supplying validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development and research. This anti-ZNF416 antibody supports investigations into mechanobiology and transcriptional regulation, and we can assist with custom reagent sourcing and technical consultation to meet your unique project needs.