Anti-ZNF350 Rabbit Polyclonal Antibody for WB, ELISA - Q9GZX5

Core Product Specifications and Parameters

Biological Background: ZNF350 Function and Localization

• ZNF350, also known as ZBRK1 and ZFQR, is a KRAB-containing zinc finger protein that functions as a transcriptional repressor.
• It specifically binds the DNA sequence 5'-GGGxxxCAGxxxTTT-3' located within intron 3 of the GADD45 gene, suggesting a role in DNA damage response regulation.
• The protein localizes to the nucleus and nuclear matrix, consistent with its function in transcriptional regulation.
• ZNF350 is widely expressed across human tissues.
• Structurally, it contains multiple C2H2-type zinc finger domains mediating DNA binding and an N-terminal KRAB domain essential for transcriptional repression.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of ZNF350 is ~60 kDa; use nuclear extracts to enrich the target protein and validate band specificity with appropriate controls.
• The immunogen spans amino acids 285–532, which includes part of the zinc finger region; this region is conserved, and the antibody may cross-react with mouse ZNF350 as indicated—validate reactivity in your experimental system.
• In ELISA applications, consider using the recombinant immunogen or nuclear extracts as coating antigen to assess antibody titer and specificity.
• When probing DNA damage responses, monitor GADD45 expression changes in parallel as a functional readout.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, academic laboratories, and research institutes with high-quality IVD raw materials, covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For ZNF350-related projects, we offer tailored sourcing support—whether you need rare-target raw materials or custom antibody development, our one-stop service bridges your R&D and production needs.